The Carrel patch clamp for renal transplantation
نویسندگان
چکیده
منابع مشابه
Automated planar patch-clamp.
Ion channels are integral membrane proteins that regulate the flow of ions across the plasma membrane and the membranes of intracellular organelles of both excitable and non-excitable cells. Ion channels are vital to a wide variety of biological processes and are prominent components of the nervous system and cardiovascular system, as well as controlling many metabolic functions. Furthermore, i...
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Patch-clamp recording has enabled single-cell electrical, morphological and genetic studies at unparalleled resolution. Yet it remains a laborious and low-throughput technique, making it largely impractical for large-scale measurements such as cell type and connectivity characterization of neurons in the brain. Specifically, the technique is critically limited by the ubiquitous practice of manu...
متن کاملPatch-Clamp Evidence for Calcium Channels
To test the hypothesis that Ca channel plays a role in renal epithelial Ca transport, we exposed and patched apical membranes of freshly microdissected rabbit connecting tubules (CNTs). Single channel Ca currents were recorded with Ba as the charge carrier. In the cell-attached mode, 8-Br-cAMP increased the open-state probability (P.) to 0.6%. In excised, inside-out patches, P. was low spontane...
متن کاملWhole-Cell Patch-Clamp Recordings
The patch-clamp recording technique, which measures ionic currents under voltage clamp, was designed to study small patches of membranes in which near perfect control of the transmembrane voltage can be achieved readily. The recent application of patch-clamp methodology to the analysis of whole-cell current actually defies many of the original design requirements. Nevertheless, whole-cell recor...
متن کاملExploration of the Basolateral Chloride Channels in the Renal Tubule using the Patch-Clamp Technique
Chloride channels located on the basolateral membrane are known to be involved in chloride absorption in several parts of the renal tubule, and particularly in the thick ascending limb and distal convoluted tubule. The data available suggest that the ClC-K channels play the major role in this process. We provide here a description of the electrophysiological properties of these channels, still ...
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ژورنال
عنوان ژورنال: The Annals of The Royal College of Surgeons of England
سال: 2017
ISSN: 0035-8843,1478-7083
DOI: 10.1308/rcsann.2017.0080